Vitamin B12 (Cobalamin, Methylcobalamin)

Method Description

The determination of water-soluble vitamins in food and dietary supplements using Ultra-Performance Liquid Chromatography with Diode Array Detection (UPLC-DAD) involves several steps. Firstly, sample preparation is carried out to extract the water-soluble vitamins from the matrix. Then, the extracted samples are injected into the UPLC system equipped with a Diode Array Detector (DAD). The UPLC separates the individual vitamins based on their chemical properties and elution times. The DAD detects the presence of the vitamins by measuring their absorbance at specific wavelengths. Calibration curves prepared using standard solutions allow for quantification of the vitamins in the samples. This method offers high sensitivity, precision, and efficiency for analyzing water-soluble vitamins in complex matrices.